Now researchers in the Kaila Lab at DBB have discovered new functional principles underlying proton and sodium transport in Mbh from the hyperthermophilic archaeon Pyrococcus furiosus.

The researchers identified Na+ binding sites and proton pathways leading across the membrane and to the NiFe-active center, as well as conformational changes that control the ion uptake. They also discovered mechanistic principles, shared with the modern members of the mitochondrial complex I-superfamily. This study, recently published in JACS, provides new insight into the molecular principles of archaeal energy transduction.


M.E. Mühlbauer, A.P. Gamiz-Hernandez, V.R.I. Kaila
Functional Dynamics of an Ancient Membrane-Bound Hydrogenase.
JACS 2021 Nov 30.
doi: 10.1021/jacs.1c09356.

Figure. Structure and functional principles of the membrane-bound hydrogenase from Pyrococcus furiosus. From: Mühlbauer et al., JACS, 2021 (in press).